Ngaba i-dihydrocoumarin inetyhefu?

I-Dihydrocoumarin, ivumba elimnandi, elisetyenziswa ekutyeni, likwasetyenziswa endaweni ye-coumarin, lisetyenziswa njengencasa yokuhombisa; I-blend cream, incasa yekhokhonathi, nesinamon; Ikwasetyenziswa njengencasa yecuba.

Ngaba i-dihydrocoumarin inetyhefu?

I-Dihydrocoumarin ayinabungozi. I-Dihydrocoumarin yimveliso yendalo efumaneka kwi-yellow vanilla rhinoceros. Ilungiswa nge-hydrogenation ye-coumarin xa kukho i-nickel catalyst kwi-160-200 ℃ kwaye iphantsi koxinzelelo. Ingasetyenziswa njengezinto eziluhlaza, ezifakwe kwi-alkaline aqueous solution ukuvelisa i-o-hydroxyphenylpropionic acid, ukuphelelwa ngamanzi emzimbeni, kunye ne-closed-loop efunyenweyo.

Imeko yokugcina

Ivaliwe kwaye imnyama, igcinwe kwindawo epholileyo neyomileyo, indawo engaphakathi kwibhareli incinci kangangoko phantsi kweemvume zokhuseleko, kwaye izaliswe lukhuseleko lwe-nitrogen. Gcina kwindawo yokugcina izinto epholileyo, enomoya. Hlala kude nomlilo, amanzi. Kufuneka igcinwe yodwa kwi-oxidizer, ungayixubi indawo yokugcina. Ixhotyiswe ngeentlobo ezahlukeneyo kunye nobungakanani bezixhobo zomlilo ezifanelekileyo.

Uphononongo lwe-in vitro

Uvavanyo lwe-in vitro enzyme, i-dihydrocoumarin ibangele ukuthintelwa kwe-SIRT1 (IC50 ye-208μM) okuxhomekeke kuxinzelelo. Ukuncipha komsebenzi we-SIRT1 deacetylase kubonwe nakwiidosi ze-micromolar (85±5.8 kunye ne-73± 13.7% yomsebenzi kwi-1.6μM kunye ne-8μM, ngokulandelelana). I-Microtubule SIRT2 deacetylase nayo ithintelwe ngendlela efanayo exhomekeke kumthamo (IC50 ye-295μM).

Emva kweeyure ezingama-24 zokuchatshazelwa, i-dihydrocoumarin (1-5mM) yonyuse ubuthi kwimigca yeeseli ze-TK6 ngendlela exhomekeke kwidosi. I-Dihydrocoumarin (1-5mM) yonyuse i-apoptosis kwimigca yeeseli ze-TK6 ngendlela exhomekeke kwidosi ngexesha leeyure ezi-6. Idosi ye-5mM ye-dihydrocoumarin yonyuse i-apoptosis kwixesha leeyure ezi-6 kumgca weeseli ze-TK6. Emva kwexesha lokuchatshazelwa kwimizuzu engama-24, i-dihydrocoumarin (1-5mM) yonyuse i-p53 lysine 373 kunye ne-382 acetylation ngendlela exhomekeke kwidosi kumgca weeseli ze-TK6.