Ngaba i-dihydrocoumarin inetyhefu

I-Dihydrocoumarin, i-fragrance, esetyenziswa ekutyeni, ekwasetyenziswa njenge-coumarin substitute, esetyenziswa njenge-flavour cosmetic; Umxube we-cream, i-coconut, i-sinamon flavour; Ikwasetyenziswa njengencasa yecuba.

Ngaba i-dihydrocoumarin inetyhefu

I-Dihydrocoumarin ayinayo ityhefu. I-Dihydrocoumarin yimveliso yendalo efumaneka kwi-yellow vanilla rhinoceros. Ilungiselelwe yi-hydrogenation ye-coumarin phambi kwe-nickel catalyst kwi-160-200 ℃ naphantsi koxinzelelo. Ingaphinda isetyenziswe njengento ekrwada, i-hydrolyzed kwisisombululo se-alkaline samanzi ukuvelisa i-o-hydroxyphenylpropionic acid, ukuchithwa kwamanzi, i-loop evaliweyo efunyenweyo.

Imeko yokugcina

Ivaliwe kwaye imnyama, igcinwe kwindawo epholileyo neyomileyo, indawo kwi-barrel incinci kangangoko kunokwenzeka phantsi kweemvume zokhuseleko, kwaye igcwele ukukhuselwa kwe-nitrogen. Gcina kwindawo epholileyo nengena umoya. Hlala kude nomlilo, amanzi. Kufuneka igcinwe ngokwahlukileyo kwi-oxidizer, musa ukuxuba ukugcinwa. Ixhotyiswe ngeentlobo ezihambelanayo kunye nobuninzi bezixhobo zomlilo.

Isifundo se-in vitro

I-in vitro i-enzymatic assay, i-dihydrocoumarin yenza inhibition exhomekeke kwi-concentration-dependent ye-SIRT1 (IC50 ye-208μM). Ukunciphisa umsebenzi we-SIRT1 we-deacetylase kwabonwa nakwiidosi ze-micromolar (85±5.8 kunye ne-73± 13.7% umsebenzi kwi-1.6μM kunye ne-8μM, ngokulandelanayo). I-Microtubule SIRT2 i-deacetylase nayo yavinjelwa ngendlela efanayo exhomekeke kwidosi (IC50 ye-295μM).

Emva kweeyure ze-24 zokuvezwa, i-dihydrocoumarin (i-1-5mM) inyuse i-cytotoxicity kwimigca yeseli ye-TK6 ngendlela exhomekeke kwidosi. I-Dihydrocoumarin (i-1-5mM) inyuse i-apoptosis kwimigca yeseli ye-TK6 ngendlela exhomekeke kwi-dose kwixesha le-6 yeyure. I-dose ye-5mM ye-dihydrocoumarin inyuse i-apoptosis kwixesha leeyure ze-6 kwi-cell line ye-TK6. Emva kwexesha lokuvezwa kweeyure ezingama-24, i-dihydrocoumarin (1-5mM) yanda i-p53 lysine 373 kunye ne-382 i-acetylation ngendlela exhomekeke kwi-dose kwi-cell line ye-TK6.